Journal: Scientific Reports

Article Title: Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts

doi: 10.1038/s41598-020-59058-4

Figure Lengend Snippet: Graphs depicting co-localized detection of intracellular coagulation factors with Golgi and ER proteins. Intensities were measured from red and green channels along a single line in merged images of HUVECs stained simultaneously with antibodies against coagulation proteins and antibodies either to Golgi or PDI. The intensities were measured along the white line shown on the merged images (left panels) and the corresponding graphs (right panels). Fluorescence intensity is on the y-axis and distance (in pixels) is on the x-axis. ( a ) Prothrombin (red) plus PDI (green), line = 10 µm ( b ) FVII (red) plus Golgi (green), line = 7 µm ( c ) FIX (red) plus Golgi (green), line = 11 µm and ( d ) FX (red) plus PDI (green), line = 9 µm. HUVECs were imaged at 100× and nuclei were detected with DAPI. Images were selected from 4–16 similar images for each coagulation protein. The specific detection antibodies are listed in the Fluorescence colocalization measurement section of the Methods. The region of interest (ROI) is the line where the intensities were measured.

Article Snippet: The degree of similarity between detection in the red and green channels in merged images was determined using the fluorescence colocalization module within IP Lab software (Scanalytics, Inc.).

Techniques: Coagulation, Staining, Fluorescence